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KMID : 0606920080160040385
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Biomolecules & Therapeutics
2008 Volume.16 No. 4 p.385 ~ p.393
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Anti-inflammatory Effects of the Methanol Extract of Polytrichum Commune via NF-κB Inactivation in RAW 264.7 Macrophage Cells
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Cho Woong
Park Seung-Jae
Shin Ji-Sun
Noh Young-Su
Cho Eu-Jin
Nam Jung-Hwan
Lee Kyung-Tae
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Abstract
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As an attempt to search for bioactive natural products exerting anti-inflammatory activity, we evaluated the effects of the methanol extract of Polytrichum commune Hedw (PCM) (Polytrichaceae) on lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin () and pro-inflammatory cytokines release in murine macrophage cell line RAW 264.7. PCM potently inhibits the production of NO, , tumor necrosis factor (TNF)- and interleukin (IL)-6. Consistent with these results, PCM also concentration-dependently inhibited LPS-induced inducible NO synthase (iNOS) and cyclooxygase (COX)-2 at the protein levels, and iNOS, COX-2, TNF- and IL-6 at the mRNA levels without an appreciable cytotoxic effect on RAW 264.7 macrophag cells. Furthermore, PCM inhibited LPS-induced nuclear factor-kappa B (NF-B) activation as determined by NF-B reporter gene assay, and this inhibition was associated with a decrease in the nuclear translocation of p65 and p50 NF-B. Taken together, these results suggest that PCM may play an anti-inflammatory role in LPS-stimulated RAW 264.7 macrophages through the inhibitory regulation of iNOS, COX-2, TNF- and IL-6 via NF-B inactivation.
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KEYWORD
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Polytrichum commune, Inducible nitric oxide synthase (iNOS), Cyclooxygenase-2 (COX-2), Nuclear factor- kappa B (NF-B), Anti-inflammation
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